兔抗CHEK2(Phospho-Thr383) 多克隆抗體

Background:

In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1.

Applications:

WB, IF

Name of antibody:

CHEK2(Phospho-Thr383)

Immunogen:

Peptide sequence around phosphorylation site of threonine383 (M-R-T(p)-L-C) derived from Human Chk1.

Full name:

checkpoint kinase 2

Synonyms：

CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

SwissProt:

O96017

WB Predicted band size:

61 kDa

WB Positive control:

COS7 cells lysates treated with UV

WB Recommended dilution:

500-1000

IF positive control:

Hela cells

IF Recommend dilution:

100-200