克隆類型: mouse monoclonal
技術(shù)規(guī)格
Background: |
The blue fluorescent protein (BFP) is a mutant of the green fluorescent protein, where the phenolic ring of the chromophore has been replaced by imidazole cycle of histidine residue. The usability of BFP as a fluorescent marker is hampered by its low fluorescence quantum yield at room temperature. The intensity of fluorescence increases by a factor of 4.5 when the temperature is decreased from 320 K down to 225 K. The fluorescence is also enhanced by hydrostatic pressure. Both effects have been explained by shift of the equilibrium between hydrogen nonbonded and hydrogen-bonded chromophores. |
Applications: |
WB |
Name of antibody: |
mBFP |
Immunogen: |
This antibody is raised against a recombinant protein and recognizes mBFP. |
Full name: |
monomeric blue fluorescence protein |
SwissProt: |
P80893 |
WB Predicted band size: |
26 kDa |
WB Positive control: |
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY mBFP ne) cDNA for 48 hrs and lysed |
WB Recommended dilution: |
1000-5000 |