小鼠抗CSK單克隆抗體

Background:

Non-receptor tyrosine-protein kinase that plays an important role in the regulation of cell growth, differentiation, migration and immune response. Phosphorylates tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) including LCK, SRC, HCK, FYN, LYN or YES1. Upon tail phosphorylation, Src-family members engage in intramolecular interactions between the phosphotyrosine tail and the SH2 domain that result in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane via binding to transmembrane proteins or adapter proteins located near the plasma membrane. Suppresses signaling by various surface receptors, including T-cell receptor (TCR) and B-cell receptor (BCR) by phosphorylating and maintaining inactive several positive effectors such as FYN or LCK.

Applications:

WB, IF

Name of antibody:

CSK

Immunogen:

Fusion protein of human CSK

Full name:

C-terminal Src kinase

SwissProt:

P41240

WB Predicted band size:

51 kDa

WB Positive control:

Jurkat and C6 cell lysates

WB Recommended dilution:

1000-5000

IF positive control:

C6 cells

IF Recommend dilution:

10-50